MO331LINEAGE TRACING OF REGENERATING PROXIMAL TUBULE CELLS (STC) BY SINGLE CELL PROFILING IN ACUTE KIDNEY INJURY

Abstract Background and Aims Acute tubular injury accounts for the most common intrinsic cause acute kidney (AKI). The scattered cell (STC) phenotype was discovered as a uniform reaction of tubule cells triggered by injury. Our group first to identify an inducible transgenic mouse (PEC-rtTA-mouse) specifically labeling STCs with eGFP. Analysis transcriptional factors associated signaling pathways might reveal function role in AKI. Method Here, we performed single-cell RNA sequencing unilateral ischemia-reperfusion murine model AKI 8, 24, 48 hours 6 12 days after induction. Results Genes expressing proximal proteins transporters were markedly downregulated during transition into STC upon injury; but expression recovered over time resolution re-differentiated cells. This provides evidence that is transient reversible Among phenotype, could 2 sub-clusters; highly proliferating sub-cluster cycle analysis showed highest proportion cycling second eGFP-positive cluster appeared very early expressed distinct set genes (defined 7 anchor genes). Some up-regulated are known markers hence confirming specificity our line. Conclusion study gene patterns repair at multiple points suggests